Unveiling Cell Fate: Exploring Apoptosis and Necrosis with the Annexin Fluorescent Dye 488 Labelled / PI Apoptosis Detection Kit

The Annexin Fluorescent Dye 488 Labelled / PI Apoptosis Detection Kit

The Annexin Fluorescent Dye 488 Labelled / PI Apoptosis Detection Kit (AffiASSAY) is a specialized assay kit designed to identify and quantify apoptotic cells within a cell population. This kit utilizes annexin V conjugated with a fluorescent dye, typically FITC or Alexa Fluor 488, to bind to phosphatidylserine (PS) residues exposed on the outer membrane of apoptotic cells. Additionally, propidium iodide (PI) is used as a counterstain to label necrotic cells with compromised membrane integrity. The AffiASSAY version ensures high sensitivity, precision, and reliability in detecting apoptotic and necrotic cells, providing valuable insights into cell death processes and apoptotic pathways.

Dual Staining

The kit utilizes annexin V labeled with a fluorescent dye (typically FITC or Alexa Fluor 488) to detect apoptotic cells by binding to phosphatidylserine (PS) residues on the cell membrane. Propidium iodide (PI) is used to label necrotic cells with compromised membrane integrity. This dual staining approach allows for the simultaneous detection of apoptotic and necrotic cells in the same sample.

Quantitative Analysis

The kit enables quantitative analysis of apoptotic and necrotic cells using flow cytometry or fluorescence microscopy, allowing for precise measurement of cell death rates and apoptotic indices.


AffiASSAY provides good value for money, offering an affordable solution for routine apoptosis detection assays in research laboratories.  


The Annexin Fluorescent Dye 488 Labelled / PI Apoptosis Detection Kit (AffiASSAY) finds its best applications in various research areas, including

Drug Discovery and Development

It is used to assess the efficacy and toxicity of candidate drugs or compounds by detecting apoptotic and necrotic cells in response to treatments. This aids in identifying potential therapeutic agents and evaluating their safety profiles.

Cancer Research

The kit is invaluable for studying cancer cell death mechanisms and evaluating the effectiveness of chemotherapy or targeted therapies. It allows researchers to quantify apoptosis and necrosis in tumor cells, providing insights into drug resistance and treatment response.

Cell Biology

AffiASSAY is used to investigate apoptotic pathways, signaling molecules, and regulatory mechanisms in basic cell biology research. It enables researchers to elucidate the molecular mechanisms underlying cell death processes and cellular stress responses.


It is employed in immunology research to study apoptosis in immune cells, such as lymphocytes, macrophages, and dendritic cells. This helps understand immune cell turnover, activation-induced cell death, and immune system regulation.


The kit is utilized in neuroscience studies to investigate apoptosis and necrosis in neurons and glial cells under various conditions, including neurodegenerative diseases, brain injury, and neurotoxicity.


AffiASSAY is used in toxicology studies to assess the cytotoxic effects of chemicals, environmental pollutants, and industrial compounds on cells. It provides valuable data for evaluating potential health risks and regulatory compliance.

Stem Cell Research

It aids in evaluating apoptosis and cell death during stem cell differentiation, reprogramming, and transplantation. This helps optimize culture conditions and enhance the efficiency of stem cell-based therapies.

Infectious Disease Research

The kit is used to investigate apoptosis in host cells during viral, bacterial, or parasitic infections. It helps understand host-pathogen interactions and immune responses to infectious agents.

Overall, the Annexin Fluorescent Dye 488 Labelled / PI Apoptosis Detection Kit (AffiASSAY) serves as a versatile tool for detecting and quantifying apoptosis and necrosis in various cell types and research contexts, facilitating a deeper understanding of cell death mechanisms and disease processes.

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