Overview
AffiGEN's 20S Immunoproteasome Activity Assay Kit uses a plate-based fluorometric assay to measure the activity of the 20S immunoproteasome. In this assay, the 20S immunoproteasome cleaves a fluorogenic substrate to release 7-amino-4-methylcoumarin, a fluorescent compound that produces a stable signal at Ex/Em = 345/445 nm. The kit can detect as low as 0.02 µU (0.02 pmol substrate proteolysis per minute) of 20S immunoproteasome under assay conditions.
AffiASSAY® 20S Immunoproteasome Activity Assay Kit (Fluorometric)
The kit can detect as low as 0.02 µU (0.02 pmol substrate proteolysis per minute) of 20S immunoproteasome under assay conditions., A plate-based fluorometric assay to measure the activity of the 20S immunoproteasome.
Alternative Name
20S Immunoproteasome Activity Kit (Fluorometric), 20S Immunoproteasome Assay Kit (Fluorometric), 20S Immunoproteasome Activity Assay Kit, 20S Immunoproteasome Activity Assay (Fluorometric)
Species Reactivity
All
Applications
To measure the activity of 20S immunoproteasome in samples.
Tag Line
To measure the activity of the 20S immunoproteasome
Detection Method
Fluorescence (Ex/Em = 345/445 nm)
Sample Type
Purified protein, Cell lysate, Tissue lysate (e.g. spleen tissue)
Storage Conditions
-20°C
Shipping Conditions
Gel Pack
Shelf life
12 Months