Product Overview
The AffiASSAY® Acetate Colorimetric Assay Kit provides a robust and highly sensitive method for the quantification of acetate in biological samples. Designed for research laboratories, this assay combines enzymatic specificity with a stable chromogenic readout, enabling reliable detection in the low nanomole range.
The assay is optimized for 96-well plate format, allowing high-throughput workflows with excellent reproducibility.
Assay Principle
Acetate is quantified through a multi-step enzymatic cascade:
Conversion of acetate into an activated intermediate via a specific enzymatic module
Coupled phosphorylation reaction generating a measurable cofactor change
Reduction of a chromogenic probe producing a stable colored signal
Signal measured at 450 nm, proportional to acetate concentration
The internal chemistry is optimized to maximize sensitivity while minimizing background interference.
Kit Contents
Component | Function |
Reaction Buffer | Optimized assay environment |
Enzymatic Reaction Mix | Drives analyte conversion and signal generation |
Cofactor System | Enables coupled enzymatic reactions |
Chromogenic Detection Reagent | Produces measurable color signal |
Acetate Standard | Calibration reference |
Components are provided in stabilized formats for optimal performance and shelf life.
Applications
- Metabolic pathway analysis
- TCA cycle and energy metabolism studies
- Microbial fermentation monitoring
- Cell culture metabolite profiling
- Nutritional and biochemical research
Key Features & Benefits
- High Sensitivity – Detects acetate at nanomole levels
- Robust & Reproducible – Optimized enzymatic cascade
- Simple Workflow – Mix-and-read format
- Flexible Sample Types – Cells, tissues, biological fluids
- 96-Well Compatible – Ideal for high-throughput screening
- Low Background Interference – Engineered reaction system
Performance Specifications
- Detection Method: Colorimetric
- Wavelength: 450 nm
- Format: Microplate (96-well)
- Sensitivity: < 1 nmol per well
- Assay Time: ~30–60 minutes
Sample Compatibility
- Cell lysates
- Tissue extracts
- Serum / plasma
- Culture media
- Other biological fluids
Samples should be clarified prior to use to ensure optimal performance.
General Workflow
- Prepare standards and samples
- Add to microplate wells
- Add reaction master mix
- Incubate at room temperature
- Measure absorbance at 450 nm
- Calculate concentration using standard curve
Detailed protocol provided in the technical datasheet.
Storage & Stability
- Store at -20°C upon receipt
- Keep enzymatic components cold during use
- Avoid repeated freeze-thaw cycles
Important Considerations
- Environmental acetate contamination may affect results
- Highly metabolically active samples may require dilution
- Background correction is recommended for complex matrices
Technical Documentation
- Datasheet available upon request
- Validation data and application support available
Ask for technical file by email to info@affigen.com.