Overview
AffiGEN’s Acyl-CoA Synthetase Fluorometric assay kit provides a rapid, sensitive and straightforward way to measure ACS activity in various samples. In the assay, acyl-CoA produced by ACS activity is metabolized by the Enzyme Mix, Developer Mix and Converter Mix to generate an intermediate compound, which reacts with a probe, yielding a fluorescent signal that can be measured with excitation at 535 nm and emission at 587 nm. This assay can detect ACS activity as low as 5 mU/µl in various samples.
AffiASSAY® Acyl-CoA Synthetase Fluorometric Assay Kit
N/A
Alternative Name
Acyl-CoA Synthetase Assay Kit, ACS, Acyl-CoA Synthetase Fluorometric Assay Kit, Acyl-CoA Synthetase Fluorometric Assay, Acyl-CoA Synthetase Fluorometric Kit, ACS Fluorometric Assay, Acyl-CoA Synthetase Kit, ACS Fluorometric Kit, ACS Fluorometric Assay Kit
Species Reactivity
All
Applications
Measurement of Acyl-CoA Synthetase activity in various tissues/cells
Tag Line
To detect ACS activity in various samples
Detection Method
Fluorescence (Ex/Em 535/587 nm)
Sample Type
Purified enzyme preparations, Animal tissues: Liver, intestine etc., Cell culture: Adherent or suspension cells
Storage Conditions
-20°C
Shipping Conditions
Gel Pack
Shelf life
12 Months