Overview
AffiGEN’s AMP Colorimetric Assay Kit provides a convenient method to detect AMP in biological samples. In this assay, AMP is converted to pyruvate in the presence of Pyrophosphate and Phosphoenolpyruvate. This is followed by a set of enzymatic reactions to generate a colored product with a strong absorbance at 570 nm. The absorbance is proportional to the amount of AMP present in samples. The kit is rapid, sensitive, easy to use and high-throughput adaptable. It can measure AMP level lower than 10 µM in various sample types.
AffiASSAY® AMP Colorimetric Assay Kit
N/A
Alternative Name
AMP Assay Kit, AMP Colorimetric Kit, Adenosine Monophosphate Assay, Adenosine Monophosphate Kit, Adenosine Monophosphate Colorimetric Assay, AMP Colorimetric Assay, Adenosine Monophosphate, AMP Colorimetric Assay Kit, AMP Assay, Adenosine Monophosphate Colorimetric Kit, AMP
Species Reactivity
All
Applications
Study the regulation of AMPK by AMP, Mechanistic study of key cellular processes such as Ca2+ signaling, glucose uptake, lipid uptake, etc., Measurement of AMP level in various cell or tissue types
Tag Line
To detect AMP in biological samples
Detection Method
Absorbance, OD 570 nm
Sample Type
Adherent or suspension Cells: e.g. HeLa, HEK239, Jurkat cells, Tissue lysates: e.g. Liver, Kidney, etc.
Storage Conditions
-20°C
Shipping Conditions
Gel Pack
Shelf life
12 Months