Overview
Arylamine N-acetyltransferases (NATs, EC 2.3.1.5) are a group of cytosolic, conjugating enzymes involved in phase II xenobiotic metabolism. They transfer an acetyl group from acetyl Coenzyme A to a xenobiotic acceptor substrate. There are two main isoforms, NAT1 and NAT2, which share about 81% amino acid sequence homology. NAT1 is widely expressed in all tissues whereas NAT2 is present primarily, in the liver and intestine. In addition to its role in xenobiotic metabolism, NAT1 plays an important role in folate metabolism. NAT2 is a polymorphic enzyme wherein the slow acetylator phenotype of NAT2 has been linked to urinary bladder cancer whereas the rapid acetylator phenotype has been linked to colorectal cancer. The slow acetylator phenotype has also been widely linked with toxicity due to isoniazid: a widely used tuberculosis drug. AffiGEN’s Arylamine N-acetyltransferase Activity Assay Kit is a simple, one step plate based assay that measures a fluorescent product. It can measure both NAT1 and NAT2 activity and can detect as low as 1 µU in samples.
AffiASSAY® Arylamine N-acetyltransferase Activity Assay Kit (Fluorometric)
N/A
Alternative Name
Arylamine N-acetyltransferase Fluorometric Activity Assay, Arylamine N-acetyltransferase, Arylamine N-acetyltransferase, Arylamine N-acetyltransferase Fluorometric Kit, Arylamine N-acetyltransferase Fluorometric Kit, Arylamine N-acetyltransferase Activity Assay Kit, Arylamine N-acetyltransferase Activity Kit, Arylamine acetyltransferase, Arylamine N-acetyltransferase Activity Kit, Arylamine N-acetyltransferase Fluorometric Activity Kit, Arylamine N-acetyltransferase Fluorometric Assay, Arylamine N-acetyltransferase Assay Kit (Fluorometric), Arylamine N-acetyltransferase Activity Assay Kit (Fluorometric), Arylamine N-acetyltransferase Assay, Arylamine N-acetyltransferase Activity Kit (Fluorometric), Arylamine N-acetyltransferase Activity Assay, Arylamine N-acetyltransferase Fluorometric Assay Kit, Arylamine N-acetyltransferase Fluorometric Activity Assay Kit, Arylamine N-acetyltransferase Activity Assay (Fluorometric)
Species Reactivity
Mammalian
Applications
Measurement of NAT activity in tissue samples or purified protein
Tag Line
Arylamine acetyltransferase Activity Assay
Detection Method
Fluorescence (Ex/Em = 360/440 nm)
Sample Type
Purified protein, Recombinant enzyme, S9 fractions
Storage Conditions
Upon arrival, store the kit at -20°C, protected from light.
Shipping Conditions
Gel Pack
Shelf life
12 Months