AffiASSAY® Arylamine N-acetyltransferase Activity Assay Kit (Fluorometric)

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CAT# AFG-BV-0073
Size: 100 Assays

648.00 648.0 USD 648.00

648.00

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Overview

Arylamine N-acetyltransferases (NATs, EC 2.3.1.5) are a group of cytosolic, conjugating enzymes involved in phase II xenobiotic metabolism. They transfer an acetyl group from acetyl Coenzyme A to a xenobiotic acceptor substrate. There are two main isoforms, NAT1 and NAT2, which share about 81% amino acid sequence homology. NAT1 is widely expressed in all tissues whereas NAT2 is present primarily, in the liver and intestine. In addition to its role in xenobiotic metabolism, NAT1 plays an important role in folate metabolism. NAT2 is a polymorphic enzyme wherein the slow acetylator phenotype of NAT2 has been linked to urinary bladder cancer whereas the rapid acetylator phenotype has been linked to colorectal cancer. The slow acetylator phenotype has also been widely linked with toxicity due to isoniazid: a widely used tuberculosis drug. AffiGEN’s Arylamine N-acetyltransferase Activity Assay Kit is a simple, one step plate based assay that measures a fluorescent product. It can measure both NAT1 and NAT2 activity and can detect as low as 1 µU in samples.

AffiASSAY® Arylamine N-acetyltransferase Activity Assay Kit (Fluorometric)

N/A

Alternative Name

Arylamine N-acetyltransferase Fluorometric Activity Assay, Arylamine N-acetyltransferase, Arylamine N-acetyltransferase, Arylamine N-acetyltransferase Fluorometric Kit, Arylamine N-acetyltransferase Fluorometric Kit, Arylamine N-acetyltransferase Activity Assay Kit, Arylamine N-acetyltransferase Activity Kit, Arylamine acetyltransferase, Arylamine N-acetyltransferase Activity Kit, Arylamine N-acetyltransferase Fluorometric Activity Kit, Arylamine N-acetyltransferase Fluorometric Assay, Arylamine N-acetyltransferase Assay Kit (Fluorometric), Arylamine N-acetyltransferase Activity Assay Kit (Fluorometric), Arylamine N-acetyltransferase Assay, Arylamine N-acetyltransferase Activity Kit (Fluorometric), Arylamine N-acetyltransferase Activity Assay, Arylamine N-acetyltransferase Fluorometric Assay Kit, Arylamine N-acetyltransferase Fluorometric Activity Assay Kit, Arylamine N-acetyltransferase Activity Assay (Fluorometric)

Species Reactivity

Mammalian

Applications

Measurement of NAT activity in tissue samples or purified protein

Tag Line

Arylamine acetyltransferase Activity Assay

Detection Method

Fluorescence (Ex/Em = 360/440 nm)

Sample Type

Purified protein, Recombinant enzyme, S9 fractions

Storage Conditions

Upon arrival, store the kit at -20°C, protected from light.

Shipping Conditions

Gel Pack

Shelf life

12 Months