AffiASSAY® Beta-Mannosidase Activity Assay Kit (Fluorometric)

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CAT# AFG-BV-0097
Size: 100 Assays

620.00 620.0 USD 620.00

620.00

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    Overview

    Beta-mannosidase (β-Man; EC 3.2.1.25) is an enzyme that hydrolyzes terminal, non-reducing beta-D-mannose residues in beta-D-mannosides. It is a member of the glycoside hydrolase family 2. There are numerous applications of β-Man including the synthesis of glycosides, production of fermentable sugars, curing mannosidosis, etc. Lysosomal β-Man is essential for catabolizing the Asn-linked glycans of glycoproteins thereby maintaining the cellular homeostasis. β-Man deficiency results in Beta-Mannosidosis, a rare inherited disorder affecting the break-down of mannose-containing disaccharides. These disaccharides gradually accumulate in the lysosomes and cause cells to malfunction. AffiGEN’s Beta-Mannosidase Activity Assay Kit provides a facile, rapid way to monitor total β-Man activity in various biological samples. In this kit, β-Man cleaves a synthetic specific substrate and releases a fluorophore, which can be easily quantified (Ex/Em = 360/445 nm). The substrate is specific to β-Mannosidase and can differentiate from α-Mannosidase. The assay is simple, sensitive and can detect as low as 1.0 µU of beta-mannosidase activity.

    AffiASSAY® Beta-Mannosidase Activity Assay Kit (Fluorometric)

    Can detect as low as 1.0 µU of beta mannosidase activity.,The substrate is specific to β-Man and can differentiate from β-mannosidase,A facile, rapid way to monitor total Beta-Mannosidase in various biological samples.

    Alternative Name

    Beta-Mannosidase Activity Assay,Beta-Mannosidase Activity Assay Kit,EC 3.2.1.25,Mannosidase Activity Assay,b-Mannosidase Activity Assay Kit,Beta-Mannosidase Activity Kit,β-Man,Mannosidase Activity Assay Kit,Beta-Mannosidase Activity Assay (Fluorometric),Beta-Mannosidase Activity Kit (Fluorometric),b-Mannosidase Activity Assay Kit (Fluorometric),Beta-Mannosidase Activity Assay Kit (Fluorometric)

    Species Reactivity

    All

    Applications

    Measurement of beta-mannosidase activity in various samples

    Tag Line

    To monitor total β-Mannosidase activity in various biological samples

    Detection Method

    Fluorescence detection Ex/Em = 360/445 nm

    Sample Type

    Cell Lysates: 3T3 cells, etc.,Biological fluids: Human plasma, etc.

    Storage Conditions

    -20°C

    Shipping Conditions

    Gel Pack

    Shelf life

    12 Months

    DataSheet & SDS available under request

    Contact info@affigen.com or call  (800) 660-1620

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