Overview
L-Citrulline is a non-proteogenic, semi-essential amino acid. Citrulline is formed either by ornithine carbamoyltransferase or as a byproduct of the Nitric oxide synthase (NOS) activity. Citrulline generated in the NOS reaction can be recycled to arginine by the two enzymes argininosuccinate synthetase (ASS) and argininosuccinate lyase (ASL) acting in the Urea Cycle. Citrulline concentrations have been shown to be elevated in patients with mutated ASS or ASL genes. Watermelon is one of the richest ources of Citrulline. Additionally, Citrulline has been advertised as sports nutrition supplement due to its involvement in Nitric Oxide (NO) synthesis, which helps with vasodilation. AffiGEN’s Citrulline Assay Kit provides a rapid, specific, and easy method for the measurement of total Citrulline concentrations in a wide variety of samples. In this enzymatic assay, Citrulline is converted into a series of intermediates, which further reacts with a probe producing a stable fluorescent signal (Ex/Em = 535/587 nm). The kit is simple, easy to perform, sensitive and is highthroughput adaptable. It can detect as low as 2 µM Citrulline in biological samples.
AffiASSAY® Citrulline Fluorometric Assay Kit
Easy method for the measurement of total Citrulline concentrations in a wide variety of samples., Specific, Rapid
Alternative Name
Citrulline Fluorometric Assay, Citrulline Assay Kit, Citrulline Fluorometric Kit, Citrulline Fluorometric Kit, Citrulline Kit, Citrulline Assay, L-Citrulline, Citrulline, Citrulline Fluorometric Assay Kit, L-Citrulline
Species Reactivity
All
Applications
Analysis of Urea cycle and NO cycle, Measurement of Citrulline in beverages and biological samples.
Tag Line
for measuring total Citrulline concentrations
Detection Method
Fluorescence (Ex/Em = 535/587 nm)
Sample Type
Fruit juices, Biological fluids such as serum
Storage Conditions
-20°C
Shipping Conditions
Gel Pack
Shelf life
12 Months