Overview
• Detection method - Fluorescence (Ex/Em 406/468 nm)
• Species reactivity - Eukaryotes
• Applications -
• Rapid assessment of native/recombinant CYP1A2 activity in lysates or microsomal fractions prepared from tissues, cells.
• Screening of drugs and novel ligands for interaction with native/recombinant CYP1A2.
AffiASSAY® Cytochrome P450 1A2 (CYP1A2) Activity Assay Kit (Fluorometric)
• Simple, convenient, highly sensitive
• Fluorescent assay enables easy determination of CYP1A2 activity in a variety of biological samples
• The substrate shows low background and a high signal-to-noise ratio
• Kit includes CYP1A2 inhibitor (α-naphthoflavone) and a stable, recombinant human CYP1A2 co-expressed with NADPH Reductase as a positive control
Alternative Name
CYP1A2 Activity Assay kit
Species Reactivity
Eukaryotes
Applications
• Rapid assessment of native/recombinant CYP1A2 activity in lysates or microsomal fractions prepared from tissues, cells.
• Screening of drugs and novel ligands for interaction with native/recombinant CYP1A2.
Tag Line
Highly Sensitive; Includes human CYP1A2 - NADPH Reductase as positive control.
Detection Method
Fluorescence (Ex/Em 406/468 nm)
Sample Type
• Human liver microsomes and liver S9 fractions
• Lysates of tissues and cultured cells, primary hepatocytes
• Heterologously expressed recombinant CYP2C preparations
Storage Conditions
-20°C
Shipping Conditions
Gel Pack
Shelf life
12 Months