AffiASSAY® Cytochrome P450 3A4 (CYP3A4) Activity Assay Kit (Fluorometric)

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CAT# AFG-BV-0246
Size: 200 Assays

606.00 606.0 USD 606.00

606.00

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Overview

• Detection method - Fluorescence (Ex/Em 535/587 nm)

• Species reactivity - Eukaryotes

• Applications -

• Rapid assessment of native/recombinant CYP3A4 activity in lysates or microsomal fractions prepared from tissues, cells.

• Screening of drugs and novel ligands for interaction with native/recombinant CYP3A4.

AffiASSAY® Cytochrome P450 3A4 (CYP3A4) Activity Assay Kit (Fluorometric)

• Simple, convenient, highly sensitive

• Fluorescent assay enables determination of CYP3A4, CYP3A5 activity in a variety of biological samples

• The substrate shows low background and a high signal-to-noise ratio

• Kit includes CYP3A inhibitor ketoconazole and a stable, recombinant human CYP3A4 co-expressed with NADPH Reductase as a positive control


Alternative Name

N/A

Species Reactivity

Eukaryotes

Applications

• Rapid assessment of native/recombinant CYP3A4 activity in lysates or microsomal fractions prepared from tissues or cells.

• Screening of drugs and novel ligands for interaction with native/recombinant CYP3A4.

Tag Line

Highly Sensitive; Includes human CYP3A4 - NADPH Reductase as positive control.

Detection Method

Fluorescence (Ex/Em 535/587 nm)

Sample Type

• Human liver microsomes and liver S9 fractions

• Lysates of tissues and cultured cells, primary hepatocytes

• Heterologously expressed recombinant CYP3A4 preparations

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

Shelf life

12 Months