Overview
AffiGEN’s DPPH Antioxidant Assay Kit is a high-throughput adaptable, microplate-based assay that allows the rapid quantification of antioxidant capacity of many samples including foods, beverages, biological fluids (e.g. human serum), etc. In this assay, DPPH free radical, which is deep blue in color abstracts a hydrogen atom in a one-electron reaction to form 2,2-diphenyl-1-picrylhydrazine (DPPH-H), which is pale yellow in color. The color change is associated with a decrease in absorbance at 517 nm. The decrease in absorbance is proportional to the antioxidant capacity of the sample. The synthetic antioxidant, Trolox is included in the kit and is used to standardize the sample antioxidant capacity relative to Trolox (Trolox Equivalents Antioxidant Capacity, TEAC).
AffiASSAY® DPPH Antioxidant Assay Kit (Colorimetric)
A high-throughput adaptable, microplate-based assay that allows the rapid quantification of antioxidant capacity of many samples including foods, beverages, biological fluids (e.g. human serum), etc.
Fast and convenient
Alternative Name
DPPH Assay Kit
DPPH Antioxidant Assay Kit
Antioxidant Assay Kit
Species Reactivity
All
Applications
Rapid quantification of antioxidant activity in beverages, foods, plant extracts, biological fluids i.e. human serum, etc.
Tag Line
For rapid quantification of antioxidant activity in various samples
Detection Method
Absorbance at 517 nm
Sample Type
Food and beverages (fruits, berries, tea, etc.)
• Plant and herbal extracts
• Biological fluids (i.e. serum)
Storage Conditions
4ºC
Shipping Conditions
Gel Pack
Shelf life
12 Months