Overview
In AffiGEN’s Glutamate Decarboxylase Activity Assay Kit, GAD decarboxylases L-Glutamate in the presence of Pyridoxal Phosphate to produce CO₂ and GABA intermediates. The intermediates then go through a series of enzymatic reactions to form NADPH, which converts the non-fluorescent probe to a fluorescent product measured at Ex/Em = 535/587 nm. The activity of GAD is proportional to the fluorescent signal. AffiGEN’s Glutamate Decarboxylase Activity Assay Kit offers a rapid, simple, sensitive, and reliable test for detecting GAD activity as low as 0.01 µU in samples.
AffiASSAY® Glutamate Decarboxylase Activity Assay Kit (Fluorometric)
Reliable test for detecting GAD activity as low as 0.01 µU in samples, Sensitive, Rapid, Simple
Alternative Name
Glutamate Decarboxylase Activity Assay Kit (Fluorometric), EC 4.1.1.15, GAD, Glutamate Decarboxylase Activity Kit, Glutamate Decarboxylase Activity, Glutamate Decarboxylase Assay Kit, Glutamate Decarboxylase Activity, Glutamate Decarboxylase, Glutamate Decarboxylase Activity Assay, Glutamate Decarboxylase Activity Kit (Fluorometric), Glutamate Decarboxylase Assay, Glutamate Decarboxylase Activity Assay (Fluorometric), Glutamate Decarboxylase Activity Assay (Fluorometric), Glutamate Decarboxylase Activity Assay Kit
Species Reactivity
All
Applications
Mechanistic study of GAD, Measure GAD activity in plant tissues (e.g. spinach, germinated barley seeds etc.), Measure GAD activity in animal tissues (e.g. mouse brain, mouse pancreas etc.), Measure GAD activity of pure enzyme
Tag Line
For detecting GAD activity
Detection Method
Fluorescence (Ex/Em = 535/587 nm)
Sample Type
Animal tissues: Brain and Pancreas, Plant tissues: e.g. Spinach and Germinated Barley Seeds
Storage Conditions
-20°C
Shipping Conditions
Gel Pack
Shelf life
12 Months