Overview
• Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) for Triglycerides and Microscopy for Histochemical staining of Lipid droplets Applications:
• Evaluate hepatic steatosis, quantify triglycerides, and visualize lipid droplet accumulation
• Screen drug candidates for steatotic risk
AffiASSAY® Hepatic Steatosis Assay Kit
• Only Kit on the market to use a dual method to study Hepatic Steatosis: Quantifies Triglycerides (colorimetric/fluorometric) and visualizes lipid droplets by staining with Oil Red O.
• The kit provides enough reagents for 100 assays for triglyceride quantitation and staining reagents enough for two 96-well plates, two 6-well plates, or four 100 mm culture dishes.
• Easy and simple protocol. This kit can be used to screen and evaluate steatosis risk of drug candidates and to study the mechanism of steatosis development.
• Chloroquine is included as a positive control for inducing steatosis in liver cells, such as HepG2.
Alternative Name
N/A
Species Reactivity
Mammalian
Applications
• Evaluate hepatic steatosis, quantify triglycerides, and visualize lipid droplet accumulation
• Screen drug candidates for steatotic risk
Tag Line
Only Kit on the market to use a dual method to study Hepatic Steatosis: Quantifies Triglycerides (colorimetric/fluorometric) and visualizes lipid droplets by staining with Oil Red O.
Detection Method
Absorbance (OD 570 nm) or Fluorescence (Ex/Em = 535/587 nm) and Microscopy
Sample Type
Liver cells such as HepG2
Storage Conditions
4°C
Shipping Conditions
Gel Pack
Shelf life
12 Months