AffiASSAY® Hydroxy Acid Oxidase Activity Assay Kit (Fluorometric)

Overview

Hydroxy Acid Oxidase (HAO) (EC 1.1.3.15), also known as glycolate oxidase is a flavin mononucleotide dependent enzyme that oxidizes alpha hydroxy acid to oxo acid. Glycolate is the primary substrate for HAO, which gets oxidized to glyoxalate. HAO contributes to the general pathway of fatty acid α-oxidation and plays an important role in ethylene glycol poisoning. It is expressed in liver, kidney and pancreas. The most well studied enzyme of this family is the plant glycolate oxidase that has an essential role in photorespiration. AffiGEN’s Hydroxy Acid Oxidase Activity Kit is a simple plate-based fluorometric assay for measuring HAO activity in biological samples. HAO oxidizes hydroxy carboxylate, which is accompanied by the release of H2O2. H2O2 then oxidizes a non-fluorescent probe to a fluorescent product in the presence of enzyme mix via an enzymatic reaction. The assay can detect as low as 1 µU of HAO activity in biological samples.