AffiASSAY® Hydroxy Acid Oxidase Activity Assay Kit (Fluorometric)

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CAT# AFG-BV-0396
Size: 100 Assays

572.00 572.0 USD 572.00

572.00

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Overview

Hydroxy Acid Oxidase (HAO) (EC 1.1.3.15), also known as glycolate oxidase is a flavin mononucleotide dependent enzyme that oxidizes alpha hydroxy acid to oxo acid. Glycolate is the primary substrate for HAO, which gets oxidized to glyoxalate. HAO contributes to the general pathway of fatty acid α-oxidation and plays an important role in ethylene glycol poisoning. It is expressed in liver, kidney and pancreas. The most well studied enzyme of this family is the plant glycolate oxidase that has an essential role in photorespiration. AffiGEN’s Hydroxy Acid Oxidase Activity Kit is a simple plate-based fluorometric assay for measuring HAO activity in biological samples. HAO oxidizes hydroxy carboxylate, which is accompanied by the release of H2O2. H2O2 then oxidizes a non-fluorescent probe to a fluorescent product in the presence of enzyme mix via an enzymatic reaction. The assay can detect as low as 1 µU of HAO activity in biological samples.

AffiASSAY® Hydroxy Acid Oxidase Activity Assay Kit (Fluorometric)

The assay can detect as low as 1 µU of HAO activity in biological samples., Simple plate-based fluorometric assay for measuring HAO activity in biological samples.

Alternative Name

Hydroxy Acid Oxidase Activity Assay (Fluorometric), Hydroxy Acid Oxidase Activity Assay Kit, HAO Activity Assay Kit, HAO Activity Kit, Hydroxy Acid Oxidase Assay Kit (Fluorometric), Hydroxy Acid Oxidase Activity Assay, Hydroxy Acid Oxidase Activity Assay, Hydroxy Acid Oxidase Activity Kit (Fluorometric), HAO Assay Kit (Fluorometric), HAO Activity Kit (Fluorometric), HAO Activity Assay, HAO Activity Assay Kit (Fluorometric), Hydroxy Acid Oxidase Activity Kit, Hydroxy Acid Oxidase Activity Kit

Species Reactivity

All

Applications

Measurement of HAO activity

Tag Line

for measuring HAO activity in biological samples

Detection Method

Fluorescence (Ex/Em = 535/587 nm)

Sample Type

Purified protein, Cell lysate, Recombinant enzyme, Plant tissue lysate, Animal tissue lysate (Liver tissue)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

Shelf life

12 Months