AffiASSAY® Iodide Colorimetric Assay Kit

Overview

Iodide (I−) is a halide anion and a necessary and limiting substrate for thyroid hormone synthesis. This essential element enables the thyroid gland to produce thyroid hormones, thyroxine (T4) and triiodothyronine (T3). Additionally, Iodide is important in many essential biological processes such as basal metabolism, temperature regulation, intellectual development for children, muscular development, normal heart function, growth of skeleton etc. Food is the main source of daily supply of iodide in humans. Iodide can be found in grains, fish, dairy products, fruits, iodized salts etc. Iodine deficiency affects around two billion people worldwide. Iodide deficiency can cause diseases such as goiter, depression, fatigue, hypothyroidism, intellectual disability, delayed sexual development etc. However, excess intake of Iodide is also harmful and can inhibit the release of thyroid hormones and cause inflammation of the thyroid gland and even thyroid cancer. AffiGEN’s Iodide Colorimetric Assay Kit provides a quick, convenient, non-radioactive method to determine Iodide concentration in various sample types including foods, liquid and biological samples. The assay relies on the Iodide ion as a specific catalyst to convert a yellow colored substrate to a colorless product. The reduction in absorbance signal at 330 nm is directly proportional to the Iodide concentration in the samples. Most common ions (eg. Na+, Ca2+, Cu2+, K+, mg2+, Fe3+, NH4+, CO32- Br-, Cl-, SO42-, NO3- and PO43-) do not interfere with the assay. The kit is simple, rapid, sensitive and can detect as low as 0.2 μM Iodide under the assay conditions.