Overview
Methionine Adenosyltransferases (MATs, EC 2.5.1.6), also known as Adenosylmethionine Synthetases are a family of enzymes that synthesize S-adenosyl-L-methionine (SAMe) from L-methionine and ATP. SAMe is a primary biochemical alkylation agent (one of only two possible methyl group donors) and is also a vital metabolic precursor of the transsulfuration and polyamine synthesis (aminopropylation) pathways. All organisms express at least one MAT enzyme. In mammals, three isozymes of MAT have been identified. MAT1 and MAT3 isozymes are predominantly expressed in the liver, whereas MAT2A is expressed in most tissues. Upregulation of the MAT2A isozyme has been linked to several human diseases. MAT2A has become a popular drug target for novel cancer therapeutics as well as for hepatic fibrosis and non-alcoholic fatty liver disease. AffiGEN’s Methionine Adenosyltransferase Activity Assay Kit enables the rapid measurement of MAT activity in complex biological matrices. The assay is based on the detection of pyrophosphate, which is generated stoichiometrically during the generation of SAMe. Pyrophosphate is enzymatically metabolized to an intermediate product, which reacts with the probe to form a stable chromophore that is detected by absorbance at 570 nm. The assay is homogeneous, simple to perform and does not require complicated sample processing. The assay is high-throughput adaptable and has a limit of quantification of 2 mU MAT activity per well.
AffiASSAY® Methionine Adenosyltransferase Activity Assay Kit (Colorimetric)
Does not require complicated sample processing,The limit of quantification of 2 mU MAT activity per well,Simple to perform,The assay is high-throughput adaptable,The assay is homogeneous
Alternative Name
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Species Reactivity
All
Applications
Rapid assessment of MAT activity in biological samples or recombinant MAT preparations
Tag Line
Measurement of MAT activity in complex biological matrices
Detection Method
Absorbance 570 nm
Sample Type
Human or animal soft tissue (i.e. brain, liver, lung, etc.) homogenates,Cultured cell lysates (adherent or suspension cells),Heterologously expressed recombinant MAT preparations
Storage Conditions
-20°C
Shipping Conditions
Gel Pack
Shelf life
12 Months