Overview
AffiGEN Myristoylated Protein Assay Kit is a highly specific, simple and robust tool for labeling and detection of myristoylated proteins. In this assay, an Alkynyl Myristic Acid is added directly to the cells that get incorporated into proteins during or post translation followed by the click reaction with the azide-containing dye. This system offers a powerful method for imaging localization, trafficking and dynamics of Myristoylated proteins, or detection by FACS for quantitative studies. Labeled Myristoylated Proteins can be directly detected in 1D or 2D gels using the appropriate excitation sources, or enriched by immunoprecipitation with biotin-azide or antibodies prior to proteomic analysis. We provide sufficient materials for 100 assays in a 96-well plate format.
AffiASSAY® Myristoylated Protein Assay Kit, Red Fluorescence
N/A
Alternative Name
Myristoylated Protein Assay Kit, Myristoylated Protein Assay, Myristoylated Protein Assay, Myristoylated Protein Assay, Red Fluorescence, Myristoylated Protein Assay Kit, Red Fluorescence, Myristoylated Protein Kit, Red, NMT, Myristoylated Protein Assay Kit, Red Fluorescence, EZClick™, Myristoylated Protein Kit, Red Fluorescence, Myristoylated Protein Assay Kit, Myristoylated Protein Assay, Red, Myristoylated Protein Assay Kit
Species Reactivity
Mammalian
Applications
Detection and quantification of biosynthesis, dynamics and turnover of myristic acid modified proteins, Identification and localization of myristic acid modified proteins, Screening for genotoxic compounds and effectors of protein modifications
Tag Line
for labeling and detection of myristoylated proteins
Detection Method
FACS and Microscopy
Sample Type
Suspension or adherent cell cultures
Storage Conditions
-20°C
Shipping Conditions
Gel Pack
Shelf life
12 Months