Overview
AffiGEN’s NADH Oxidase Activity Assay Kit couples oxidation of NADH by NOX and reduction of a colorless probe to a brightly colored product generating fluorescence at Ex/Em = 535/587 nm. The fluorescence generated is directly proportional to the NOX activity in samples. The kit can be used to determine NOX activity in a variety of sample type with a detection limit of ~ 15 µU of NOX activity per reaction.
AffiASSAY® NADH Oxidase Activity Assay Kit (Fluorometric)
Simple, Rapid, Can be used to determine NOX activity in a variety of sample type with a detection limit of ~ 15 µU of NOX activity per reaction.
Alternative Name
NADH Oxidase Activity Assay (Fluorometric), NADH Oxidase Kit (Fluorometric), NADH Oxidase Activity Kit (Fluorometric), NADH Oxidase Activity Kit, NADH Oxidase Assay Kit, NADH Oxidase Assay Kit (Fluorometric), NADH Oxidase Activity, NADH Oxidase Activity Assay Kit, NADH Oxidase Activity Assay, NADH Oxidase Assay, NADH Oxidase
Species Reactivity
Mammalian
Applications
Analysis of ROS in different samples, Mechanistic studies of various cancers, Measurement of NADH oxidase activity in various tissues/cells, Screening anti-cancer drugs
Tag Line
to measure NADH oxidase activity
Detection Method
Fluorescence (Ex/Em = 535/587 nm)
Sample Type
Cell culture: adherent or suspension cells, Animal tissues: Liver, muscle, heart etc.
Storage Conditions
-20°C
Shipping Conditions
Gel Pack
Shelf life
12 Months