AffiASSAY® Phosphoglycerate Mutase Activity Assay Kit (Colorimetric/Fluorometric)

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(1 review)

CAT# AFG-BV-0554
Size: 100 Assays

795.00 795.0 USD 795.00

795.00

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Overview

Phosphoglycerate Mutase (PGAM) is an important enzyme in the glycolytic pathway that catalyzes the reversible conversion of 3‐phosphoglycerate to 2‐phosphoglycerate. It plays an important role in regulating glycolysis and anabolic activity to promote cancer cell proliferation. Several studies reported that PGAM is upregulated in many human cancers. Inhibition of this enzyme could lead to tumor cell death thereby making PGAM an attractive therapeutic target. AffiGEN’s PGAM Activity Assay Kit can be used to detect its activity in biological samples. The kit utilizes the ability of an active PGAM to catalyze the conversion of 3-Phosphoglycerate to 2-Phosphoglycerate, which is subsequently used to generate an intermediate product. The intermediate product then stoichiometrically reacts with an OxiRed™ probe to generate color (OD 570 nm) or fluorescence (Ex/Em = 535/587 nm). This assay kit provides a rapid, simple and sensitive method to detect PGAM activity as low as 0.05 mU in a variety of samples.

AffiASSAY® Phosphoglycerate Mutase Activity Assay Kit (Colorimetric/Fluorometric)

Rapid, Sensitive method to detect PGAM activity as low as 0.05 mU in a variety of samples., Simple

Alternative Name

Phosphoglycerate Mutase Activity Assay Kit (Colorimetric/Fluorometric), PGAM assay, PGAM, PGAM1, PGAM Activity Kit, PGAM activity assay, PGAM activity assay kit, PGAM activity kit, Phosphoglycerate Mutase Kit (Colorimetric/Fluorometric), Phosphoglycerate Mutase Activity Assay, Phosphoglycerate Mutase Kit (Colorimetric/Fluorometric), PGAM assay kit, Phosphoglycerate Mutase Activity Assay Kit, Phosphoglycerate Mutase Assay Kit (Colorimetric/Fluorometric), Phosphoglycerate Mutase Assay Kit, Phosphoglycerate Mutase Assay

Species Reactivity

All

Applications

Analysis of glycolytic metabolism in different sample types, Mechanistic studies of various cancers, Screening anti-cancer drugs, Measurement of PGAM activity in various tissues/cells

Tag Line

to detect PGAM activity in biological samples

Detection Method

Absorbance (OD 570 nm) or Fluorescence (Ex/Em = 535/587 nm)

Sample Type

Animal tissues: Liver, muscle, heart etc., Cell culture: adherent or suspension cells

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

Shelf life

12 Months