Overview
AffiGEN’s Plasminogen Activator Inhibitor-1 Activity Assay Kit is a two-step colorimetric assay. In the first step, samples are incubated with a known amount of tPA thereby allowing PAI-1 and tPA to form an inactive complex. In the second step, the residual tPA converts plasminogen to plasmin, which in turn hydrolyzes the plasmin chromogenic substrate thereby releasing pNA (chromophore). The absorbance of the released pNA is inversely proportional to the PAI-1 activity in the samples. The assay kit is simple, specific and can detect as low as 2 U/ml of PAI-1 activity in samples.
AffiASSAY® Plasminogen Activator Inhibitor-1 Activity Assay Kit (C)
Can detect as low as 2 U/ml of PAI-1 activity in samples,Specific,Simple
Alternative Name
PAI-1,PAI 1,PAI Activity Assay Kit (C),PAI-1 Activity,Plasminogen Activator Inhibitor-1 Activity Kit (C),PAI-1 Activity Assay Kit (C),PAI1 Activity Assay,PAI-1 Activity Kit (C),Plasminogen Activator Inhibitor-1 Assay Kit (C),Plasminogen Activator Inhibitor,PAI-1 Activity Assay,Plasminogen Activator Inhibitor-1 Activity Assay,PAI-1 Activity Assay,Plasminogen Activator Inhibitor-1 Activity Assay Kit,PAI 1 Activity Assay Kit (C),Plasminogen Activator Inhibitor-1,Plasminogen Activator Inhibitor Activity Assay Kit (C),Plasminogen Activator Inhibitor-1 Activity Assay Kit (C)
Species Reactivity
All
Applications
Measurement of PAI-1 activity in Biological Samples such as plasma, cell culture medium,Analysis and study of fibrinolytic system
Tag Line
To measure PAI-1 activity in Biological Samples
Detection Method
Absorbance, OD 450 nm
Sample Type
Plasma,Cell and tissue culture supernatants
Storage Conditions
-20°C
Shipping Conditions
Gel Pack
Shelf life
12 Months