AffiASSAY® Sialyltransferase Activity Assay Kit (Fluorometric)

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CAT# AFG-BV-0611
Size: 100 Assays

667.00 667.0 USD 667.00

667.00

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    Overview

    Sialyltransferases (ST, Galactosyldiacylglycerol alpha-2,3-sialyltransferase; 2.4.99.5) belong to the glycosyltransferase family and are expressed in a variety of organisms from bacteria to higher mammals. Previously, it was believed to be not expressed in plants, but sialyltransferase like proteins have been recently identified in rice. STs catalyze the transfer of sialic acid (a kind of N-acetylneuraminic acid) from CMP-Neu5Ac moieties to sugar molecules on the terminal regions of glycolipids and glycoproteins. In higher animals, terminal sialic acid residue-containing glycoconjugates have various important functions including modulation of the immune system, brain development, etc. In bacteria, they play a role in virulence and are present on the bacterial surface to protect them from the immune system. AffiGEN’s Sialyltransferase Activity Assay Kit is a simple, two-step plate based assay that measures the fluorescent product at Ex/Em = 410/470 nm. The assay consists of three enzymatic steps leading to the generation of ammonia. Ammonia then reacts with the detection reagent to form a fluorescent product measured at Ex/Em = 410/470 nm. The kit can detect activity from both α-1,6 and α-2,6 sialyltransferases and can measure as low as 20 μU ST activity under assay conditions.

    AffiASSAY® Sialyltransferase Activity Assay Kit (Fluorometric)

    The kit can measure as low as 20 μU ST activity under assay conditions,A simple, two-step plate based assay that measures the fluorescent product at Ex/Em = 410/470 nm,For measuring Sialyltransferase activity using a 96-well plate format,The kit can detect activity from both α-1,6 and α-2,6 sialyltransferases

    Alternative Name

    Sialyltransferase Activity Assay Kit,Sialyltransferase Activity Kit (Fluorometric),Sialyltransferase Assay Kit (Fluorometric),Sialyltransferase Activity,Sialyltransferase Activity Assay,Sialyltransferase Activity Assay Kit,Sialyltransferase,Sialyltransferase Assay,Sialyltransferase Kit,Sialyltransferase Activity Kit

    Species Reactivity

    All

    Applications

    Measurement of Sialyltransferase activity using a 96-well plate format

    Tag Line

    for measuring Sialyltransferase activity

    Detection Method

    Fluorescence (Ex/Em = 410/470 nm)

    Sample Type

    Recombinant enzyme,Purified protein,Tissue or cell lysate

    Storage Conditions

    -20°C

    Shipping Conditions

    Gel Pack

    Shelf life

    12 Months

    DataSheet & SDS available under request

    Contact info@affigen.com or call  (800) 660-1620

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