AffiASSAY® Streptokinase Activity Assay Kit (Colorimetric)

Overview

Human Streptokinase is a therapeutic drug and is used as a fibrinolytic agent to treat patients with acute myocardial infarction. Streptokinase (SK) is a plasminogen activator, which works by binding to plasminogen to form a catalytically active streptokinase-plasminogen enzymatic complex (Plg-SK) thereby converting plasminogen to plasmin. In nature, Streptokinase is produced by Group A and Group C Streptococci. SK helps Streptococci in hijacking plasminogen in the human fibrinolytic system thereby dissolving the host fibrin barriers and facilitating bacterial spreading. SK is listed in the World Health Organization (WHO) Model List of essential medicines and is a leading fibrinolytic agent in the treatment of thromboembolic conditions. It is cost-effective and is easy to produce. AffiGEN’s Steptokinase Activity Assay Kit can be used to evaluate Streptokinase produced in bulk culture and its effect on plasminogen activation in serum. The assay is simple, easy to perform and can be completed in 3 easy steps. The assay can detect as little as 3 mU of Streptokinase Activity in less than 60 min. The overall reaction is as follows: Plasminogen + SK Plg-SK SK + Plasmin Plasmin + SK Substrate Plasmin-SK Substrate Plasmin + pNA (Absorbance 405 nm)