Overview
AffiGEN’s Transketolase Activity Assay Kit is a simple, plate-based fluorometric assay for measuring TKT activity in biological samples. In this assay, TKT transfers a two-carbon group from a donor keto sugar to an acceptor aldose sugar. The product formed converts a non-fluorescent probe to a fluorescent product via an enzymatic reaction in the presence of a developer and an enzyme mix. The assay can detect as low as 5 µU of TKT activity in biological samples.
AffiASSAY® Transketolase Activity Assay Kit (Fluorometric)
Plate-based fluorometric assay, Simple, The assay can detect as low as 5 µU of TKT activity in biological samples
Alternative Name
Transketolase Activity Assay Kit (Fluorometric), Transketolase Assay, Transketolase Kit (Fluorometric), Transketolase Activity Assay Kit, Transketolase Activity, Transketolase Activity Assay Kit, Transketolase Activity Assay (Fluorometric), Transketolase Activity Kit, TKT, Transketolase Kit, Transketolase Activity Kit (Fluorometric), Transketolase, Transketolase Activity Assay, Transketolase Activity (Fluorometric)
Species Reactivity
All
Applications
Measure Transketolase activity
Tag Line
for measuring TKT activity in biological samples
Detection Method
Fluorescence (Ex/Em = 535/587 nm)
Sample Type
Purified protein, Tissue lysate (e.g. Liver tissue), Cell lysate, Recombinant enzyme
Storage Conditions
-20°C
Shipping Conditions
Gel Pack
Shelf life
12 Months