Overview
The Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the gel, creating a cell-free zone. Following cell seeding the gel is removed, allowing migratory cells to move across the area and close the gap.
Product Line
Cell Based Assays.
Host
N/A.
Species Reactivity
N/A.
Shipping Temperature
Room Temp.
Product Classification
Cell Based Assays; Cell Migration, Invasion and Wound Healing; Gap Closure Assays; Cell Migration Assays, 2D Gap Closure .
Detection Method
Light Microscopy.
Application
N/A.
Storage Temperature
Upon receipt, aliquot and store the Gel Removal Solution and DAPI Fluorescence Stain at -20ºC (avoid multiple freeze/thaw cycles), and transfer the Fixation Solution to 4ºC. All other kit components should be stored at room temperature until the kit’s expiration date.