Overview
Hematopoietic stem cells (HSCs) are well-characterized, tissue-specific stem cells that are responsible for the lifelong maintenance of the hematopoietic system. HSCs or hematopoietic progenitors known as colony-forming cells (CFCs) proliferate to form discrete colonies when cultured in a suitable 3D environment, such as methylcellulose supplemented with nutrients and cytokines. Our96-Well Hematopoietic Colony Forming Cell Assay promotes the formation of HSC colonies in just 7-10 days. Cells can then be either quantified in a fluorescence plate reader or recovered from the semisolid medium for further downstream analysis.
Product Line
Stem Cell Research.
Host
N/A.
Species Reactivity
N/A.
Shipping Temperature
Blue Ice.
Product Classification
Stem Cell Research; Hematopoietic CFC Assays .
Detection Method
Fluorometric.
Application
N/A.
Storage Temperature
Store theMethylcellulose Medium at -20ºC. If the methylcellulose medium will not be used all at once, it should be aliquoted to avoid multiple freeze/thaw cycles. Store all other components at 4ºC.