Overview
Our NAD+/NADH Assay Kit detects NAD+ and NADH in cell and tissue lysates. Total NAD+/NADH can be detected or samples can be treated with an acid or base treatment to specifically detect NAD+ or NADH. This assay uses an enzymatic cycling reaction that reduces NAD+ to NADH, which then reacts with a colorimetric probe and is detected with a spectrophotometric plate reader at 450nm. NAD+/NADH levels in unknown samples are calculated based on the provided NAD+ standard curve.
Product Line
Metabolism Research.
Host
N/A.
Species Reactivity
N/A.
Shipping Temperature
2 parts: 1 RT / 1 Blue Ice.
Product Classification
Metabolism Research; Cellular Metabolism; NAD+/NADH Assay.
Detection Method
Colorimetric.
Application
N/A.
Storage Temperature
Upon receipt, store the NAD Cycling Enzyme and NAD+ Standard at -80°C. Store the Colorimetric Probe at -20°C. Store the remaining components at 4°C.