Overview
Our NADP+/NADPH Assay Kit detects NADP+ and NADPH in cell and tissue lysates. Total NADP+/NADPH can be detected or samples can be treated with an acid or base treatment to specifically detect NADP+ or NADPH. This assay uses an enzymatic cycling reaction that reduces NADP+ to NADPH, which then reacts with a colorimetric probe and is detected with a spectrophotometric plate reader at 450nm. NADP+/ NADPH levels in unknown samples are calculated based on the provided NADP+ standard curve.
Product Line
Metabolism Research.
Host
N/A.
Species Reactivity
N/A.
Shipping Temperature
2 parts: 1 RT / 1 Blue Ice.
Product Classification
Metabolism Research; Cellular Metabolism; NADP+/NADPH Assay.
Detection Method
Colorimetric.
Application
N/A.
Storage Temperature
Upon receipt, store the NADP Cycling Enzyme and NADP+ Standard at -80°C. Store the Colorimetric Probe at -20°C. Store the remaining components at 4°C.