AffiASSAY® DAG (Diacylglycerol) Assay Kit

https://www.affiassay.com/web/image/product.template/688328/image_1920?unique=e869bc6

CAT# AFG-CBL-112
Size: 100 Assays

447.00 447.0 USD 447.00

447.00

For Price Contact info@affigen.com

This combination does not exist.

Terms and Conditions
30-day money-back guarantee
Shipping: 2-3 Business Days

Overview

Our DAG (Diacylglycerol) Assay Kit measures diacylglycerol in cell lysate samples by a coupled enzymatic reaction system. First, kinase is used to phosphorylate DAG in samples, yielding phosphatidic acid. Next, lipase is used to hydrolyze phosphatidic acid to glycerol-3-phosphate. The glycerol-3-phosphate product is then oxidized by glycerol-3-phosphate oxidase, producing hydrogen peroxide. The hydrogen peroxide released from this reaction reacts specifically with the kit’s Fluorometric Probe and is detected at ex. 530-560 nm/em. 585-595 nm. DAG levels in unknown samples are determined based on the provided DAG standard curve.

Product Line

Metabolism Research.

Host

N/A.

Species Reactivity

N/A.

Shipping Temperature

Dry Ice.

Product Classification

Metabolism Research; Lipoprotein Metabolism; Phospholipid Assays.

Detection Method

Fluorometric.

Application

N/A.

Storage Temperature

Store the entire kit at -80ºC. Avoid multiple freeze/thaws by aliquoting. The Fluorometric Probe is light sensitive and should be maintained in amber tubes.