AffiASSAY® DAG (Diacylglycerol) Assay Kit

https://www.affiassay.com/web/image/product.template/688328/image_1920?unique=11d470f

CAT# AFG-CBL-112
Size: 100 Assays

447.00 447.0 USD 447.00

447.00

Contact info@affigen.com for prcing

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    Terms and Conditions
    30-day money-back guarantee
    Shipping: 2-3 Business Days

    Overview

    Our DAG (Diacylglycerol) Assay Kit measures diacylglycerol in cell lysate samples by a coupled enzymatic reaction system. First, kinase is used to phosphorylate DAG in samples, yielding phosphatidic acid. Next, lipase is used to hydrolyze phosphatidic acid to glycerol-3-phosphate. The glycerol-3-phosphate product is then oxidized by glycerol-3-phosphate oxidase, producing hydrogen peroxide. The hydrogen peroxide released from this reaction reacts specifically with the kit’s Fluorometric Probe and is detected at ex. 530-560 nm/em. 585-595 nm. DAG levels in unknown samples are determined based on the provided DAG standard curve.

    Product Line

    Metabolism Research.

    Host

    N/A.

    Species Reactivity

    N/A.

    Shipping Temperature

    Dry Ice.

    Product Classification

    Metabolism Research; Lipoprotein Metabolism; Phospholipid Assays.

    Detection Method

    Fluorometric.

    Application

    N/A.

    Storage Temperature

    Store the entire kit at -80ºC. Avoid multiple freeze/thaws by aliquoting. The Fluorometric Probe is light sensitive and should be maintained in amber tubes.

    DataSheet & SDS available under request

    Contact info@affigen.com or call  (800) 660-1620

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