Overview
Our Pyruvate Assay Kit measures pyruvate in biological samples. First, pyruvate is oxidized by pyruvate oxidase, producing hydrogen peroxide. The hydrogen peroxide is then detected at ex. 530-570 nm/em. 590-600 nm using a specific fluorometric probe. Pyruvate levels in unknown samples are determined based on the provided pyruvate standard curve.
Product Line
Metabolism Research.
Host
N/A.
Species Reactivity
N/A.
Shipping Temperature
Blue Ice.
Product Classification
Metabolism Research; Cellular Metabolism.
Detection Method
Fluorometric.
Application
N/A.
Storage Temperature
Upon receipt, store the Pyruvate Standard and 10X Assay Buffer at 4°C. Store all other components at -20ºC. The Fluorometric Probe is light sensitive and must be stored accordingly. Avoid multiple freeze/thaw cycles.