AffiASSAY® NADP+/NADPH Assay Kit (Fluorometric)

https://www.affiassay.com/web/image/product.template/688331/image_1920?unique=e869bc6

CAT# AFG-CBL-115
Size: 100 Assays

499.25 499.25 USD 499.25

499.25

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Overview

Our NADP+/NADPH Assay Kit detects NADP+ and NADPH in cell and tissue lysates. Total NADP+/NADPH can be detected or samples can be treated with an acid or base treatment to specifically detect NADP+ or NADPH. This assay uses an enzymatic cycling reaction that reduces NADP+ to NADPH, which then reacts with a fluorometric probe and is detected with a fluorescence plate reader at 530-570nm excitation and 590-600nm emission. NADP+/ NADPH levels in unknown samples are calculated based on the provided NADP+ standard curve.

Product Line

Metabolism Research.

Host

N/A.

Species Reactivity

N/A.

Shipping Temperature

2 parts: 1 RT / 1 Blue Ice.

Product Classification

Metabolism Research; Cellular Metabolism; NADP+/NADPH Assay.

Detection Method

Fluorometric.

Application

N/A.

Storage Temperature

Upon receipt, store the NADP Cycling Enzyme and NADP+ Standard at -80°C. Store the Fluorometric Probe and Electron Mediator at -20°C. Store the remaining components at 4°C.