AffiASSAY® NADP+/NADPH Assay Kit (Fluorometric)
Size: 100 Assays
Our NADP+/NADPH Assay Kit detects NADP+ and NADPH in cell and tissue lysates. Total NADP+/NADPH can be detected or samples can be treated with an acid or base treatment to specifically detect NADP+ or NADPH. This assay uses an enzymatic cycling reaction that reduces NADP+ to NADPH, which then reacts with a fluorometric probe and is detected with a fluorescence plate reader at 530-570nm excitation and 590-600nm emission. NADP+/ NADPH levels in unknown samples are calculated based on the provided NADP+ standard curve.
2 parts: 1 RT / 1 Blue Ice.
Metabolism Research; Cellular Metabolism; NADP+/NADPH Assay.
Upon receipt, store the NADP Cycling Enzyme and NADP+ Standard at -80°C. Store the Fluorometric Probe and Electron Mediator at -20°C. Store the remaining components at 4°C.