Overview
Superoxide dismutase (SOD), which catalyzes the dismutation of the superoxide anion into hydrogen peroxide and molecular oxygen, is one of the most important antioxidant enzymes. SOD enzymes are classified into three groups: cytosolic CuZn-SOD, mitochondrial Mn-SOD, and extracellular Ec-SOD. Our Superoxide Dismutase Activity Assay uses a xanthine/xanthine oxidase (XOD) system to generate superoxide anions and a chromagen to produce a water-soluble formazan dye upon reduction by superoxide anions. The superoxide dismutase activity is determined as the inhibition or reduction of chromagen.
Product Line
Oxidative Stress/Damage.
Host
N/A.
Species Reactivity
N/A.
Shipping Temperature
Dry Ice.
Product Classification
Oxidative Stress/ Damage; Antioxidant Assays; Antioxidant Enzyme Activity Assays; Superoxide Dismutase (SOD) Assay .
Detection Method
Colorimetric.
Application
N/A.
Storage Temperature
Store kit components at -20ºC until their expiration dates. Avoid multiple freeze/thaws by aliquoting. Chromagen Solution is light sensitive and should be maintained in amber tubes.