AffiASSAY® Intracellular ROS Assay Kit (Green Fluorescence)

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CAT# AFG-CBL-251
Size: 96 Assays

565.75 565.75 USD 565.75

565.75

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Overview

The ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1) . In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard fluorometric plate reader.

Product Line

Oxidative Stress/Damage.

Host

N/A.

Species Reactivity

N/A.

Shipping Temperature

Room Temp.

Product Classification

Oxidative Stress/ Damage; Reactive Oxygen Species (ROS) Assays; Intracellular ROS Assay .

Detection Method

Fluorometric.

Application

N/A.

Storage Temperature

Upon receipt, store the DCFH-DA, DCF and Hydrogen Peroxide at -20ºC. Avoid multiple freeze/thaw cycles. Store the Cell Lysis Buffer at 4ºC.