Overview
The ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1) . In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard fluorometric plate reader.
Product Line
Oxidative Stress/Damage.
Host
N/A.
Species Reactivity
N/A.
Shipping Temperature
Room Temp.
Product Classification
Oxidative Stress/ Damage; Reactive Oxygen Species (ROS) Assays; Intracellular ROS Assay .
Detection Method
Fluorometric.
Application
N/A.
Storage Temperature
Upon receipt, store the DCFH-DA, DCF and Hydrogen Peroxide at -20ºC. Avoid multiple freeze/thaw cycles. Store the Cell Lysis Buffer at 4ºC.