AffiASSAY® Cellular Antioxidant Assay Kit (Green Fluorescence)

https://www.affiassay.com/web/image/product.template/688474/image_1920?unique=d8b03bf

CAT# AFG-CBL-258
Size: 192 Assays

480.25 480.25 USD 480.25

480.25

Contact info@affigen.com for prcing

    This combination does not exist.

    Terms and Conditions
    30-day money-back guarantee
    Shipping: 2-3 Business Days

    Overview

    The Cellular Antioxidant Assay Kit is a cell-based assay for measuring the activity of an exogenous antioxidant compound within adherent cells.  Cells are first cultured in a 96-well black fluorescence cell culture plate until confluent. Then the cells are pre-incubated with a cell-permeable DCFH-DA fluorescence probe dye and the bioflavonoid Quercetin, or the antioxidant sample being tested.  After a brief incubation, the cells are washed, and the reaction started by adding the Free Radical Initiator.  The Free Radical Initiator creates free radicals that convert the probe to highly fluorescent DCF.  The Quercetin inhibits the formation of free radicals, and thus DCF formation, in a concentration dependent manner.

    Product Line

    Oxidative Stress/Damage.

    Host

    N/A.

    Species Reactivity

    N/A.

    Shipping Temperature

    Room Temp.

    Product Classification

    Oxidative Stress/ Damage; Antioxidant Assays; Cellular Antioxidant Assay.

    Detection Method

    Fluorometric.

    Application

    N/A.

    Storage Temperature

    Upon receipt, store the DCFH-DA Probe (1000X) and Quercetin at -20ºC. Avoid multiple freeze/thaw cycles. Store the store the remaining components at 4ºC.

    DataSheet & SDS available under request

    Contact info@affigen.com or call  (800) 660-1620

    Your Dynamic Snippet will be displayed here... This message is displayed because you did not provided both a filter and a template to use.