Overview
Rho-associated Kinase (ROCK) mediates Rho signaling and reorganizes the actin cytoskeleton by phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility. ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr-696, which results in an increase in the phosphorylated content of the 20-kDa myosin light chain (MLC20) . Our 96-Well ROCK Activity Assay Kit uses a safe, non-radioactive format to measure the level of active Rho Kinase in cell or tissue lysates. The kit contains a strip-well plate pre-coated with recombinant MYPT1.
Product Line
Cell Signaling and Protein Biology.
Host
N/A.
Species Reactivity
N/A.
Shipping Temperature
2 parts: 1 RT / 1 Blue Ice.
Product Classification
Cell Signaling and Protein Biology; Kinase Assays; Rho Kinase (ROCK) Assays .
Detection Method
Colorimetric.
Application
N/A.
Storage Temperature
Store active ROCK-II at -80ºC, ATP Solution at -20ºC and all other kit components at 4ºC. Avoid multiple freeze/thaw cycles.