Cell Poliferation & Cytoxicity Assay Kits

Cell Proliferation & Cytotoxicity Assay Kits are specialized tools used to assess both cell growth and cell death in response to various treatments. These kits are crucial for evaluating the effects of drugs, chemicals, and other compounds on cell health and viability.

Components and Functionality

• Cell Lines: These kits use cultured cell lines or primary cells exposed to different conditions or treatments. The assays measure changes in cell number, growth rate, and cell viability.
• Assay Reagents:
  • Proliferation Indicators: Reagents that quantify cell growth or proliferation, often through metabolic activity. Common indicators include:
    • MTT: Reduced by viable cells to produce a purple formazan product, measured spectrophotometrically.
    • XTT: Converted by metabolically active cells to a soluble, colored formazan dye.
    • WST-1: Similar to XTT, it produces a colored product proportional to the number of viable cells.
  • Cytotoxicity Markers: Reagents that detect cell death or damage, such as:
    • LDH (Lactate Dehydrogenase): Released into the culture medium by damaged or lysed cells, measured colorimetrically.
    • Live/Dead Stains: Use fluorescent dyes like propidium iodide (PI) and calcein-AM to distinguish between live and dead cells.
    • Apoptosis Indicators: Detect early and late stages of apoptosis, using assays such as Annexin V/PI staining or caspase activity assays.
• Applications:
  • Drug Screening: Evaluates the effectiveness and toxicity of pharmaceutical compounds.
  • Cancer Research: Assesses the impact of treatments on cancer cell growth and survival.
  • Toxicology: Measures the toxic effects of chemicals or environmental pollutants on cell health.
  • Basic Research: Investigates cellular responses to various stimuli and conditions.

Cell Proliferation & Cytotoxicity Assay Kits provide essential insights into cellular responses to treatments, facilitating drug development, toxicology studies, and basic research into cell biology.