Fluorometric Assay Kits

Fluorometric Assay Kits are analytical tools that use fluorescent dyes or probes to measure biological or chemical reactions. These kits are renowned for their high sensitivity and specificity, making them suitable for a wide range of applications in research, clinical diagnostics, and quality control.

  • Assay Reagents:
    • Fluorescent Dyes and Probes: Chemicals that emit fluorescence when excited by specific wavelengths of light. Commonly used dyes include:
      • Fluorescein: A green fluorescent dye used in various assays for detecting nucleic acids, proteins, or other biomolecules.
      • Rhodamine: Produces red fluorescence and is used in a range of assays, including protein quantification and cell staining.
    • Fluorescent Enzyme Substrates: Substrates that produce fluorescence upon enzymatic reaction. Examples include:
      • 4-Methylumbelliferyl (4-MU): Used in enzyme assays where the hydrolysis of 4-MU produces a fluorescent product.
      • Fluorogenic Substrates: Specific to various enzymes, such as proteases or phosphatases, and generate fluorescence upon enzymatic action.
  • Applications:
    • Nucleic Acid Quantification: Measures DNA or RNA concentrations in quantitative PCR (qPCR) and other nucleic acid assays.
    • Protein Assays: Quantifies proteins using fluorescent dyes or probes specific to protein sequences or modifications.
    • Cellular Analysis: Assesses cell viability, apoptosis, and other cellular processes using fluorescent markers.
    • Enzyme Activity: Measures the activity of specific enzymes through the production of fluorescent products from substrate reactions.
  • Technical Considerations:
    • Sensitivity: Fluorometric assays offer high sensitivity and can detect low concentrations of target molecules or low levels of enzymatic activity.
    • Specificity: Proper selection of fluorescent dyes or probes is crucial to ensure specificity for the target analyte and to minimize background fluorescence.
    • Calibration and Standards: Accurate quantitation requires calibration with standards and proper assay controls to ensure reliable and reproducible results.

Fluorometric Assay Kits provide powerful tools for detecting and quantifying a wide range of biological and chemical substances with high sensitivity and specificity, making them invaluable in research and diagnostic applications.

736.00 736.0 USD
640.00 640.0 USD
AffiASSAY® Glucose Assay Kit (Fluorometric)
CAT# AFG-CBL-314
Size: 500 Assays
712.00 712.0 USD
AffiASSAY® Alcohol Assay Kit (Fluorometric)
CAT# AFG-CBL-309
Size: 100 Assays
694.00 694.0 USD
712.00 712.0 USD
748.00 748.0 USD
AffiASSAY® Acetylcholine Assay Kit (Fluorometric)
CAT# AFG-CBL-296
Size: 96 Assays
694.00 694.0 USD
AffiASSAY® Free Glycerol Assay Kit (Fluorometric)
CAT# AFG-CBL-280
Size: 100 Assays
682.00 682.0 USD
748.00 748.0 USD
AffiASSAY® Catalase Activity Assay Kit, Fluorometric
CAT# AFG-CBL-248
Size: 500 Assays
964.00 964.0 USD
AffiASSAY® Protein Carbonyl Fluorometric Assay
CAT# AFG-CBL-225
Size: 100 Assays
862.00 862.0 USD
AffiASSAY® Ammonia Assay Kit (Fluorometric)
CAT# AFG-CBL-173
Size: 200 Assays
604.00 604.0 USD
AffiASSAY® Urea Assay Kit (Fluorometric)
CAT# AFG-CBL-172
Size: 200 Assays
808.00 808.0 USD
AffiASSAY® Glutamine Assay Kit (Fluorometric)
CAT# AFG-CBL-169
Size: 200 Assays
640.00 640.0 USD
622.00 622.0 USD
AffiASSAY® Guanine Assay Kit (Fluorometric)
CAT# AFG-CBL-155
Size: 100 Assays
664.00 664.0 USD
AffiASSAY® D-Amino Acid Assay Kit (Fluorometric)
CAT# AFG-CBL-151
Size: 100 Assays
550.00 550.0 USD
640.00 640.0 USD
AffiASSAY® Lysine Assay Kit (Fluorometric)
CAT# AFG-CBL-144
Size: 100 Assays
784.00 784.0 USD
640.00 640.0 USD
AffiASSAY® Malate Assay Kit (Fluorometric)
CAT# AFG-CBL-137
Size: 100 Assays
622.00 622.0 USD
682.00 682.0 USD
AffiASSAY® L-Amino Acid Assay Kit (Fluorometric)
CAT# AFG-CBL-118
Size: 100 Assays
550.00 550.0 USD
AffiASSAY® NADP+/NADPH Assay Kit (Fluorometric)
CAT# AFG-CBL-115
Size: 100 Assays
748.00 748.0 USD
AffiASSAY® NAD+/NADH Assay Kit (Fluorometric)
CAT# AFG-CBL-114
Size: 100 Assays
748.00 748.0 USD
AffiASSAY® Starch Assay Kit (Fluorometric)
CAT# AFG-CBL-110
Size: 100 Assays
640.00 640.0 USD
AffiASSAY® Glycogen Assay Kit (Fluorometric)
CAT# AFG-CBL-108
Size: 100 Assays
640.00 640.0 USD
664.00 664.0 USD
AffiASSAY® Lactate Assay Kit (Fluorometric)
CAT# AFG-CBL-102
Size: 100 Assays
616.00 616.0 USD
640.00 640.0 USD
1,066.00 1066.0 USD
1,018.00 1018.0 USD
1,018.00 1018.0 USD
802.00 802.0 USD
736.00 736.0 USD
1,492.00 1492.0 USD
862.00 862.0 USD
862.00 862.0 USD
862.00 862.0 USD
736.00 736.0 USD
736.00 736.0 USD
766.00 766.0 USD
1,282.00 1282.0 USD
736.00 736.0 USD
826.00 826.0 USD
634.00 634.0 USD